The analyses of the samples were carried out from 3 months to 10 months from the day of production. Pour this into a third glass, and add 10 drops of any citrus fruit juice. Record the number of drops required to titrate each solution in Data table 1. The class can work in groups to study many fruits and vegetables, and pool their data to come up with food tables for vitamin C. Vitamin C ascorbic acid is an antioxidant that is essential for human nutrition. Differences in color also make possible differences in vitamin C content. Safety Data Sheet: Iodine Potassium Iodide Solution.
The longer the juice was left in the open the more oxygen and sunlight that could decrease the content of vitamin C. The procedural method was solid and straightforward. Leftover amounts of the vitamin leave the body through the urine. The nutrient is important for the human body and helps boost immune systems. Students plot results from five different concentration standards on a best-fit graph which is used as a calibration curve for the analyses of Vitamin C in a variety of common beverages.
Although vitamin C is not naturally present in grains, it is added to some fortified breakfast cereals. The constants control variables are the amount of orange juice, the concentration of iodine and the temperature of the environment, which will remain at room temperature. In fact, when people get colds, they consume large amounts of vitamin C to try to fight off the cold. If you start with a known concentration of iodine, and carefully measure the amount of the iodine solution that you add, you can calculate how much ascorbic acid was present. The average drops at 25°C were 14 and the average at 100°C was 4 showing a decrease in 10 drops. It is also necessary to repeat each cycle of the experiment to minimise any variances in the solutions being tested. Nineteen per cent of the patients had sensory-motor axonal neuropathy and 0% had carpal tunnel syndrome.
It can be seen from the results table and graph of this experiment that the lower the temperature the more vitamin C is present in the orange juice. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine. Fruits and vegetables provide us carbohydrates, proteins and various other important organic compounds. Oranges and Vitamin C Vitamin C, or ascorbic acid, is the nutrient found in citrus fruits like oranges, grapefruit and lemons. To evaluate neuromuscular signs and symptoms in patients with newly diagnosed hypothyroidism and hyperthyroidism.
The ascorbic acid peak on the chromatogram contains contributions from the peaks of the other organic acids present. The solutions of soft drinks contain different levels of vitamin C with old and new varieties will be the independent variables in the experiment as the vitamin C content is unknown. Avoid skin and eye contact. The fresh sample yielded the largest amount of vitamin C per serving, followed by the frozen sample, the carton sample, and then the plastic jug sample. Figure 1 Air-Oxidation of Ascorbic Acid This study tested different types of commercially available natural orange juice samples to compare the amount of vitamin C in each type of container. Six different brands of tetrapak packaged citrus fruit juice were selected for this study after a market survey of tetrapak packaged citrus fruit juice commercially available in Nigerian markets. It may have probability to lose it is chemical properties such as vitamin C content.
To determine the standard curve of vitamin C based on concentrate of vitamin C. It is expected that increasing the temperature of the fruit juices will decrease the amount vitamin C in fruit juices. Do Different Types of Orange Juice Contain the Same Amounts of Vitamin C? Therefore, it is recommended to eat fruits and vegetables as raw as possible. Count the number of drops needed to change the color and record on data sheet 5. Determination of Vitamin C Concentration by Titration.
It is also important to be able to accurately measure the amounts of solutions being used each time using appropriate apparatus. These small papers are chemically treated to measure the amount of Vitamin C in fruit juices, and are typically used in labs. The color change indicates that all of the substance being titrated vitamin C, or ascorbic acid has reacted with the titrating substance iodine titration solution. I made this choice because in my research I learned that vitamin C found more in orange fruit and the manufacturer of the tablet claimed 1 tablet contain 1000 mg of vitamin C. The mixture is shaked gently after the addition of each drop of the commercial orange juice. A transfer pipette or medicine dropper would work, too.
This random error maybe reduced by getting a more accurate measuring device. We must get it from food, fresh fruits, and vegetables. In this lab, you use a technique known as titration to determine how many drops of an iodine solution are needed to consume all the vitamin C in a 1 mL sample of the drink. Oxidation of vitamin C in the juices may happen. Counting droplets from a pipette is a fairly rudimentary way of calculating how much of the vitamin solution has been added to the dye. You can use another packaged lemon or lime juice, provided the amount of vitamin C is listed on the packaging. If you needed an average of 10.