Possible complications of amniocentesis include premature labor and placental bleeding. List whether each of the following substances was positive or negative for reducing sugar, as indicated by the Benedict's test. Note: Acetic anhydride-sulphuric acid reagent. Abstract This lab focused on the effects of varying temperatures on Bacterial and Fungal Amylase enzymes and their ability to break down starch into maltose using iodine. From the experiment, we can see that sunflower oil has the least lipids content, followed by corn oil and palm oil. Whereas the lipid extracted from potato chips and sunflower seeds contain unsaturated fat as there are no white solid substances float on the acetone that decanted into petri dish.
After a few minutes, we observed the color of the test tubes and recorded our observations in the table. The different level of milky colour formed in the solutions may explain the different content of lipids in the oil. Why it's done High cholesterol usually causes no signs or symptoms. Chromatography and spectra are amongst the newer techniques. When possible, patients should also stop taking any medications that may affect the accuracy of the test results. Record the color o f the tubes Table 6.
The same goes for starches except vice versa. The cloudiness formed in the first 3 test tubes are caused by the emulsion. Introduction Macromolecules are in all forms of life. Chain length also affects solubility. The actual saponification number of corn oil is 136g while the actual saponification number of palm oil is 141g, and the actual saponification number for sunflower oil is 134 g. Microwave for 25 seconds on high. Lab Purpose: Standardized Tests The purpose of testing the organic nutrients vegetable oil, glucose, starch, powdered egg white with each of the different indicators brown paper towel, benedicts, iodine, biurets was to determine the color it would turn with positive traces of the nutrient.
Fats and oils are the principal stored forms of energy in many organisms. The change in color is correlated to the first concentration of the reducing sugar. Both guidelines also recognise the importance of lipid control in diabetic patients. The fat in the potato chip is mostly on the surface of the chip from the frying process. In a dry test tube take a small amount of solution of cholesterol in chloroform. The titration can be done by adding sodium thiosulphate solution through a burette to the flask. Repeat steps 4 through 13 with the sunflower seeds.
Separation of the fatty acids from triglycerides can be achieved with caustic soda or potassium hydroxide, but is usually simply achieved by using the enzyme lipase. Interpreting Blood Test Results Blood cholesterol levels are measured in a small blood sample taken from a finger prick, or from a vein in your arm. Acute illness, high fever, starvation, or recent surgery lowers the blood cholesterol and triglyceride levels. Lipids include fats, oils, waxes, cholesterol, other sterols, and most steroids. Lipid surfactants are important to our own cellular functions, as well as useful in stabilizing specific food products.
In technical terms,saponification involves base that is hydrolysis of triglycerides,which are esters of fatty acids, to form the sodium salt ofa carboxylate. Five percent of perfectly healthy persons will have values outside of the reference range. Apo B measurement may be helpful in the presence of hypertriglyceridaemia, but care must be taken to avoid interference from lipaemic sera. Nucleic acids are used to transfer genetic information from one cell to the other. American Association for Clinical Chemistry. Carbohydrate, Carbon, Glucose 1063 Words 4 Pages Feeney Biology 9 October 16, 2012 Identifying Organic Compounds Objective: To use indicators to test for the presence of organic compounds in certain substances.
Use of Comments on Lipid Reports Pathology laboratories are encouraged to provide interpretive comments on all laboratory reports on serum lipid measurements. The result is crystallization solid fat at room temperature. As you progress further in science, you will see variations in styles of lab reports, though they all contain the same basic principles. Aftercare Aftercare following blood lipid tests includes routine care of the skin around the needle puncture. Your blood cholesterol level is related to the foods you eat or to genetic conditions passed down from other generations of family members. The nuclear membrane dissolves in Prometaphase; protein structures, kinetochores, attach to the centromeres where the microtubules, spindle fibers, attach during cell division to pull the sister chromatids apart.
The group had representation from the three major private pathology providers in Australia which are responsible for most lipid testing in the community. Cholesterol is needed by your body to maintain the health of your cells. For people who have heart disease. Label 5 test tubes with known solutions. Surfactant is a short term for surface-active agent. The length of hydrocarbon chain and the number of double bonds in the carboxylic acid portion of the fat or oil determine the properties of the resulting soap. If your results show that your cholesterol level is high, don't get discouraged.
The components of the Monster Energy drink tested were. This pattern may be seen in the metabolic syndrome, type 2 diabetes, obesity, renal impairment, hepatic impairment, intercurrent illness, drugs, and familial forms of hypertriglyceridaemia. Conditions that produce these lipoprotein abnormalities are also listed, which in conjunction with clinical information available to the laboratory can be used to generate further comments on the lipid report. Record your observations of physical appearance on Data Table: Visual Observations of Fat. Through the use of indicators we determined the presence or absence of organic compounds. Low levels of cholesterol are associated with liver failure and inherited disorders of cholesterol production. Throughout this lab we observed that the sugar fructose was positive for reducing sugar but negative for starch while sucrose was negative for both reducing sugar and starch.
Unfortunately, this potentially useful information is rarely provided. Patient Preparation To facilitate assessment and monitoring of lipid status, especially for comparison with desirable values, it is preferable to obtain a fasting specimen. This causes the veins in your arm to fill with blood. Lower thresholds are applied if the patient is symptomatic, particularly if symptoms relate to muscles or liver. Rarely, the site can become infected. Part 3: Sunflower Seeds 1.